Krampitz G, Seemayer N
Zentralbl Bakteriol B. 1979;169(5-6):456-69.
We analysed the effect of two samples of city smog extract from Bochum and Duisburg on DNA synthesis of mammalian cells in vitro. As a test system we used tissue cultures of kidney and embryonic cells from the Syrian golden hamster and monkey kidney cells from Cercopithecus aethiops. DNA synthesis of cells was measured by autoradiography using 3H-Thymidine. Both samples of city smog extract exerted a dose-dependent decrease of the rate of DNA synthesis in tissue culture cells. These alterations of nucleic acid metabolism were expressed by a reduction of DNA-synthesizing cells and by a delay of entrance of cells in DNA synthesis. High concentrations of city smog extracts induced a large number of cell necroses. Monkey kidney cells were more sensitive to the toxic action than hamster cells. Furthermore the city smog extract from Duisburg showed a stronger toxic effect than the extract from Bochum.
我们分析了来自波鸿和杜伊斯堡的两份城市烟雾提取物样本对体外培养的哺乳动物细胞DNA合成的影响。作为测试系统，我们使用了叙利亚金黄地鼠的肾组织培养物和胚胎细胞，以及埃塞俄比亚猕猴的猴肾细胞。通过使用³H-胸腺嘧啶核苷的放射自显影法来测量细胞的DNA合成。两份城市烟雾提取物样本均使组织培养细胞中的DNA合成速率呈剂量依赖性下降。核酸代谢的这些改变表现为DNA合成细胞数量减少以及细胞进入DNA合成过程的延迟。高浓度的城市烟雾提取物会诱导大量细胞坏死。猴肾细胞比仓鼠细胞对毒性作用更敏感。此外，来自杜伊斯堡的城市烟雾提取物比来自波鸿的提取物表现出更强的毒性作用。
