Choi D, Li D, Raisman G
Division of Neurobiology, National Institute for Medical Research, Mill Hill, NW7 1AA, London, UK.
J Neurosci Methods. 2002 Jun 30;117(2):167-72. doi: 10.1016/s0165-0270(02)00098-5.
Many fluorescent retrograde tracers are commercially available for neuroanatomical studies. They have been used with varying success in different models and can be very effective in the study of the facial nerve and nucleus. We compare the tracers Fast Blue (FB), Fluoro-ruby, Fluoro-emerald, Fluoro-Gold (FG), and DiI in the rat facial nucleus after application to the buccal division of the nerve. There were no significant differences between counts of cells on the left and right sides of the brain stem with any of the tracers. FB produced a lower sample variation than the other tracers, and together with DiI, demonstrated greater axonal labelling when applied to the surface of the epineurium. FB and FG resulted in strong retrograde labelling of the facial nucleus after only 2 days from injection. All the tracers produced adequate cell labelling after 1 week from nerve application. This labelling persisted for up to 8 weeks for most tracers except FG, which did not produce satisfactory labelling at 8 weeks.
许多荧光逆行示踪剂在市场上可用于神经解剖学研究。它们在不同模型中的使用效果各异，在面神经和神经核的研究中可能非常有效。我们将示踪剂快蓝（FB）、荧光红宝石、荧光祖母绿、荧光金（FG）和碘化二苯并咪唑（DiI）应用于大鼠面神经的颊支后，对大鼠面神经核中的这些示踪剂进行比较。使用任何一种示踪剂时，脑干左右两侧的细胞计数均无显著差异。FB产生的样本变异低于其他示踪剂，并且与DiI一起应用于神经外膜表面时，显示出更强的轴突标记。注射后仅2天，FB和FG就导致面神经核强烈的逆行标记。从神经应用后1周起，所有示踪剂都产生了足够的细胞标记。除FG外，大多数示踪剂的这种标记可持续长达8周，FG在8周时未产生令人满意的标记。
