Masood A Khan, Faisal S M, Mushahid M Khan, Nadeem A, Siddiqui M U, Owais M
Inter-disciplinary Biotechnology Unit, Aligarh Muslim University, India.
Biochim Biophys Acta. 2002 Aug 19;1564(1):219-26. doi: 10.1016/s0005-2736(02)00456-x.
In the present study, we demonstrated the suitability of liposomes as a method of removing plasma bilirubin in hyperbilirubinemic rats. The liposomes have innate tendency to bind with bilirubin through hydrophobic interaction. Among different types of liposomes, the positively charged liposomes were found to have maximum affinity to free bilirubin. However, the entrapment or coupling of serum albumin on the surface of egg phosphatidylcholine liposomes can render a several-fold increase in their bilirubin binding capacity. The proteoliposomes were able to preferentially bind with bilirubin even in the presence of erythrocytes. Interestingly, these liposomes were found to displace bilirubin bound on the surface of erythrocytes as well. The results of the present study further demonstrate that albumin-bearing liposomes were equally effective in removing plasma bilirubin in experimental jaundiced animals. These observations indicate that liposome-mediated selective homing of excess plasma bilirubin to the liver cells (cf. hepatocytes) may help in the development of safer strategy for the treatment of hyperbilirubinemic conditions in the model animals.
在本研究中，我们证明了脂质体作为一种去除高胆红素血症大鼠血浆胆红素方法的适用性。脂质体具有通过疏水相互作用与胆红素结合的固有倾向。在不同类型的脂质体中，发现带正电荷的脂质体对游离胆红素具有最大亲和力。然而，在卵磷脂酰胆碱脂质体表面包封或偶联血清白蛋白可使其胆红素结合能力提高数倍。蛋白脂质体即使在存在红细胞的情况下也能够优先与胆红素结合。有趣的是，这些脂质体还能置换结合在红细胞表面的胆红素。本研究结果进一步证明，携带白蛋白的脂质体在去除实验性黄疸动物血浆胆红素方面同样有效。这些观察结果表明，脂质体介导的过量血浆胆红素向肝细胞的选择性归巢可能有助于开发更安全的策略来治疗模型动物的高胆红素血症。
