Ahmad N, Arif K, Faisal S M, Neyaz M K, Tayyab S, Owais M
Inter-Disciplinary Biotechnology Unit, Aligarh Muslim University, India.
Biochim Biophys Acta. 2006 Feb;1760(2):227-32. doi: 10.1016/j.bbagen.2005.11.010. Epub 2005 Dec 19.
In the present study, we have demonstrated the suitability of microspheres in removal of plasma bilirubin from systemic circulation of hyperbilirubinemic rats. Poly (lactide co-glycolide) microspheres (PLGA microspheres) have been shown to bind with bilirubin in both a concentration and time dependent manner. The binding affinity of bilirubin to microspheres was enhanced when rat serum albumin (RSA) was loaded into the microspheres. On evaluating the potential of microspheres in elimination of bilirubin from the systemic circulation, RSA bearing microspheres were found to be competent in both removing bilirubin from the systemic circulation and controlling elevated plasma levels of liver function enzymes in temporarily hyperbilirubinemic rats. On the basis of results of the present study, we suggest that microsphere-based delivery system may help in development of safe, effective and alternate strategy for the treatment of hyperbilirubinemic conditions in model animals.
在本研究中，我们已经证明了微球在从高胆红素血症大鼠的体循环中去除血浆胆红素方面的适用性。聚（丙交酯乙交酯）微球（PLGA微球）已被证明以浓度和时间依赖性方式与胆红素结合。当将大鼠血清白蛋白（RSA）载入微球时，胆红素与微球的结合亲和力增强。在评估微球从体循环中消除胆红素的潜力时，发现载有RSA的微球在从体循环中去除胆红素以及控制暂时高胆红素血症大鼠血浆中肝功能酶水平升高方面均具有能力。基于本研究的结果，我们认为基于微球的递送系统可能有助于开发用于治疗模型动物高胆红素血症的安全、有效且替代的策略。
