Valassina Marcello, Valentini Melissa, Valensin Pier Egisto, Cusi Maria Grazia
Department of Molecular Biology, Microbiology Section, University of Siena, Italy.
Diagn Microbiol Infect Dis. 2002 Jul;43(3):201-5. doi: 10.1016/s0732-8893(02)00393-0.
Acute meningitis is the most common neurologic disease that involves the central nervous system. The spectrum of infectious agents that cause neurologic infection is remarkably broad and numerous viruses are the most frequent cause of the aseptic meningitis syndrome. We applied a multiplex one-step method for the rapid detection of the genomic RNA of different neurotropic viruses: particles in the genus Enterovirus and Toscana virus, which are the most representative aetiologic agents in our country during the spring-summer period. We have evaluated the sensitivity and the specificity of the multiplex one-step test on positive controls and on RNA extracted from clinical samples harvested from 475 patients with meningitis hospitalized during the 1996-2001 period. The multiplex one-step RT-nPCR protocol allows for the detection of enterovirus and Toscana virus RNA in a single sample, by using, at the same time, a very small clinical sample volume. In our study we were able to diagnose 192 cases of meningitis by Toscana virus and 31 cases by enteroviruses out of 475 cases of meningitis utilizing the described one-step multiplex method.
急性脑膜炎是累及中枢神经系统最常见的神经系统疾病。引起神经感染的病原体种类极为广泛，众多病毒是无菌性脑膜炎综合征最常见的病因。我们应用了一种多重一步法快速检测不同嗜神经病毒的基因组RNA：肠道病毒属颗粒和托斯卡纳病毒，它们是我国春夏季最具代表性的病原体。我们评估了多重一步检测法对阳性对照以及从1996 - 2001年期间住院的475例脑膜炎患者采集的临床样本中提取的RNA的敏感性和特异性。多重一步RT - nPCR方案可通过同时使用非常少量的临床样本，在单个样本中检测肠道病毒和托斯卡纳病毒RNA。在我们的研究中，利用所描述的一步多重方法，在475例脑膜炎病例中，我们能够诊断出192例由托斯卡纳病毒引起的脑膜炎和31例由肠道病毒引起的脑膜炎。
