Valassina M, Meacci F, Valensin P E, Cusi M G
Department of Molecular Biology, Microbiology Section, University of Siena, Siena, Italy.
J Med Virol. 2000 Jan;60(1):86-90. doi: 10.1002/(sici)1096-9071(200001)60:1<86::aid-jmv14>3.0.co;2-n.
Acute meningitis is perhaps the most frequent among central nervous system infections. We report a study considering 277 cases of meningitis hospitalized in the southern Tuscany area (Italy) during the period from 1995 to 1998 investigated by tissue culture and polymerase chain reaction (PCR) methods. The cytochemical analysis of the cerebrospinal fluid samples suggested the diagnosis of aseptic meningitis, recognized as viral meningitis in 104 cases by detection of viral DNA or RNA. The results collected by tissue culture technique, available for 95 clinical samples, reported a positive isolation for only 12 cases. The viruses identified in the neurological infection were Toscana virus (81%), enterovirus (12%), mumps virus (3%), measles virus (1%), and herpes virus type 1 (3%). These data demonstrate the incisive role of the RNA viruses as the cause of meningitis, and overall the relevance of Toscana virus.
急性脑膜炎可能是中枢神经系统感染中最为常见的。我们报告一项研究，该研究考量了1995年至1998年期间在意大利托斯卡纳大区南部住院的277例脑膜炎病例，采用组织培养和聚合酶链反应（PCR）方法进行调查。脑脊液样本的细胞化学分析提示为无菌性脑膜炎诊断，通过检测病毒DNA或RNA在104例中确诊为病毒性脑膜炎。组织培养技术收集的结果，95份临床样本可用，仅报告12例阳性分离。在神经感染中鉴定出的病毒为托斯卡纳病毒（81%）、肠道病毒（12%）、腮腺炎病毒（3%）、麻疹病毒（1%）和1型疱疹病毒（3%）。这些数据证明了RNA病毒作为脑膜炎病因的关键作用，总体而言显示了托斯卡纳病毒的相关性。
