Pickles K, Pirie R S, Rhind S, Dixon P M, McGorum B C
Wellcome Trust Centre for Comparative Respiratory Research, Easter Bush Veterinary Centre, Roslin, Midlothian, UK.
Equine Vet J. 2002 May;34(3):297-301. doi: 10.2746/042516402776185967.
Bronchoalveolar lavage fluid (BALF) samples are often subject to time delays, possibly with temperature fluctuations, between collection and processing. The aim of this study was to evaluate the effects of time, temperature and 2 different fixatives on equine BALF cytology, in order to develop guidelines for optimal equine BALF storage conditions. Total nucleated cell count (TCC), differential cell counts (DCC), absolute cell counts (ACC), cell viability, cell morphology and bacterial growth of BALF samples stored at 4, 18 (+/- addition of formalin- or alcohol-based fixatives) and 38 degrees C were monitored serially over a 72 h period. The time taken for a significant reduction in TCC and cell viability of unfixed BALF samples decreased as the storage temperature increased. There was no diagnostically significant difference in DCC or ACC over this time-course at any temperature. Unfixed BALF samples showed significant bacterial growth by 24 h at 4 degrees C, and 8 h at 18 and 38 degrees C; and poor morphology by 48 h at 4 degrees C, 24 h at 18 degrees C and 8 h at 38 degrees C. Fixed BALF samples showed poor morphology with Leishman's stain compared to unfixed samples.
支气管肺泡灌洗（BALF）样本在采集和处理之间常常会出现时间延迟，温度也可能波动。本研究的目的是评估时间、温度和两种不同固定剂对马BALF细胞学的影响，以便制定马BALF最佳储存条件的指南。对储存在4℃、18℃（±添加基于福尔马林或酒精的固定剂）和38℃的BALF样本，在72小时内连续监测其总核细胞计数（TCC）、分类细胞计数（DCC）、绝对细胞计数（ACC）、细胞活力、细胞形态和细菌生长情况。随着储存温度升高，未固定的BALF样本TCC和细胞活力显著降低所需的时间减少。在任何温度下，在此时间过程中DCC或ACC均无诊断上的显著差异。未固定的BALF样本在4℃下24小时、18℃和38℃下8小时出现显著细菌生长；在4℃下48小时、18℃下24小时和38℃下8小时细胞形态不佳。与未固定样本相比，用利什曼染色法观察固定的BALF样本时细胞形态不佳。
