Chen Jiong, Chen Jianping, Adams M J
College of Life Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou, People's Republic of China.
Arch Virol. 2002 Jun;147(6):1237-46. doi: 10.1007/s00705-001-0799-6.
Sugarcane or maize leaves with mosaic virus symptoms were collected from 13 sites in China. Sequence data showed that all 8 samples from maize contained Sugarcane mosaic virus (SCMV); complete sequences were determined from 2 samples and partial sequences (the CI coding region and the 3'-part of the genome) from the others. The 5 sugarcane samples all contained a virus tentatively described as Sorghum mosaic virus (SrMV) and in three of them SCMV was also detected; 2 SrMV sequences and the 3 SCMV ones were completely determined and partial SrMV sequences were obtained from the remaining 3 samples. The features of the complete sequences of SCMV and SrMV are described for the first time. Sequence comparisons and phylogenetic analysis showed three distinct groups of Chinese SCMV sequences (sugarcane isolates from Zhejiang province, a maize isolate from Guangdong and maize isolates from other provinces). The SrMV sequences were similar to one another (> 93% identical nucleotides); they resembled published sequences in the coat protein but were less similar in the 3'-UTR. The complete sequences of SCMV and SrMV had about 70% nucleotides identical to one another and to Maize dwarf mosaic virus (MDMV). MDMV was not detected in any of the samples.
从中国13个地点采集了出现花叶病毒症状的甘蔗或玉米叶片。序列数据显示，来自玉米的所有8个样本都含有甘蔗花叶病毒（SCMV）；从2个样本中确定了完整序列，从其他样本中获得了部分序列（CI编码区和基因组的3'部分）。5个甘蔗样本都含有一种暂时被描述为高粱花叶病毒（SrMV）的病毒，其中3个样本还检测到了SCMV；完全确定了2个SrMV序列和3个SCMV序列，并从其余3个样本中获得了部分SrMV序列。首次描述了SCMV和SrMV完整序列的特征。序列比较和系统发育分析显示，中国SCMV序列分为三个不同的组（来自浙江省的甘蔗分离株、来自广东省的一个玉米分离株和来自其他省份的玉米分离株）。SrMV序列彼此相似（核苷酸同一性>93%）；它们在外壳蛋白方面与已发表的序列相似，但在3'-UTR方面相似性较低。SCMV和SrMV的完整序列彼此之间以及与玉米矮花叶病毒（MDMV）的核苷酸同一性约为70%。在任何样本中均未检测到MDMV。
