Carey Loucinda, Mitnik Luba
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Electrophoresis. 2002 May;23(10):1386-97. doi: 10.1002/1522-2683(200205)23:10<1386::AID-ELPS1386>3.0.CO;2-M.
The paper gives a historical perspective of forensic DNA analysis and overviews existing technologies implemented in forensic laboratories for DNA profiling. Short tandem repeat analysis, mitochondrial DNA and Y-chromosome analysis are described. The review also focuses on emerging new technologies, which represent an interest for the DNA forensic community. Short tandem repeat analysis, by microelectronic chip device, electrophoretic microdevice and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry are reviewed in this context.
本文给出了法医DNA分析的历史视角,并概述了法医实验室用于DNA谱分析的现有技术。文中描述了短串联重复序列分析、线粒体DNA分析和Y染色体分析。该综述还聚焦于新兴的新技术,这些技术引起了DNA法医界的兴趣。本文在此背景下对通过微电子芯片设备、电泳微设备和基质辅助激光解吸/电离飞行时间(MALDI-TOF)质谱进行的短串联重复序列分析进行了综述。