DNA通过蛋白质和纳米孔转运的序列效应理论。

Theory of sequence effects on DNA translocation through proteins and nanopores.

作者信息

Muthukumar Murugappan

机构信息

Polymer Science and Engineering, University of Massachusetts, Amherst, MA, USA.

出版信息

Electrophoresis. 2002 May;23(10):1417-20. doi: 10.1002/1522-2683(200205)23:10<1417::AID-ELPS1417>3.0.CO;2-O.

DOI:10.1002/1522-2683(200205)23:10<1417::AID-ELPS1417>3.0.CO;2-O

PMID:12116151

Abstract

A general formula for the sequence effects on the average translocation time of a polymer passing through a narrow hole under a chemical potential gradient is provided. The utility of the general expression is illustrated by considering the diblock sequence of a polymer with two kinds of monomers. The experimental conditions required for sufficient discrimination of various sequences are addressed.

摘要

给出了在化学势梯度下聚合物通过窄孔时序列对平均转运时间影响的通用公式。通过考虑具有两种单体的聚合物的双嵌段序列来说明该通用表达式的实用性。讨论了充分区分各种序列所需的实验条件。