Ortmann R
Anat Embryol (Berl). 1975 Dec 23;148(2):109-20. doi: 10.1007/BF00315264.
According to recent research the course of the tangential fibres is gaining increasing importance for the analysis of the first signs of pathological alterations in the articular cartilage. In a model-test, parallel-running synthetic double-refracting filaments, rotated between polarisator and analysator at right angles to each other effect an extinction curve which has two maxima at 45 degrees and the minimum at 0 degrees. Crossing filaments at different angles effects curves which are specific for the different angles in such a manner that with increasing angles to 45 degrees the curves approximate to a horizontal line. On the other hand the course of the different curves make it possible to determine corresponding angles. Then the same method is used to estimate the mean crossing-angles of tangential fibres in histological sections of articular cartilage with masked collagen fibres. The method is suitable for a quantitative calculation of the crossing-angles between 0 degrees and 45 degrees, which cannot be defined by the split-line method of Hultkrantz. In contrast to the conditions of the scanning electron microscope, the integrating nature of the polarized light is advantageous because it permits analysis of a more extensive visual field. From comparisons with the test model, a considerable variety of crossing angles of tangential fibres in the cartilage can be shown to exist.
根据最近的研究，切线纤维的走向对于分析关节软骨病理改变的最初迹象越来越重要。在一个模型测试中，相互平行的合成双折射细丝，在起偏器和检偏器之间相互垂直旋转，会产生一条消光曲线，该曲线在45度处有两个最大值，在0度处有最小值。以不同角度交叉的细丝会产生特定于不同角度的曲线，使得随着与45度夹角的增加，曲线近似于一条水平线。另一方面，不同曲线的走向使得确定相应的角度成为可能。然后，使用相同的方法来估计关节软骨组织学切片中被遮蔽的胶原纤维的切线纤维的平均交叉角度。该方法适用于定量计算0度至45度之间的交叉角度，而这是Hultkrantz的裂线法无法定义的。与扫描电子显微镜的情况不同，偏振光的积分特性是有利的，因为它允许分析更广阔的视野。通过与测试模型的比较，可以证明软骨中切线纤维存在相当多种交叉角度。
