Bacchi C J, Lambros C, Ellenbogen B B, Penkovsky L N, Sullivan W, Eyinna E E, Hutner S H
Antimicrob Agents Chemother. 1975 Dec;8(6):688-92. doi: 10.1128/AAC.8.6.688.
A Leptomonas of insect origin was highly susceptible to several standard trypanocides and leishmanicides in vitro. Resistance was induced to some of these drugs; clones were isolated from each strain. Cross-resistance patterns of the clones were derived for diamidines, quinapyramine (Antrycide), acriflavin, phenanthridines, and other drugs active against trypanosomes and leishmanias. Clones tested included two each that were resistant to acriflavin, Antrycide, diminazene aceturate (Berenil), and pentamidine and one that was resistant to stilbamidine. Appreciable cross-resistance was evident for all clones. Differences were observed between clones from the same parent strain. Collateral susceptibility towards isometamidium and oxophenarsine was detected in most clone-derived populations. In clones passaged without drug to test for drug fastness, acriflavin and pentamidine clones lost resistance within 10 transfers, whereas Berenil and Antrycide clones retained considerable resistance after 20 to 30 subcultures without drug. Considerations of differences in life cycles suggest that the clone collection may be useful in screening for agents effective against leishmanias and stercorarian trypanosomes rather than against salivary trypanosomes.
一种源自昆虫的细滴虫在体外对几种标准的锥虫杀灭剂和利什曼原虫杀灭剂高度敏感。对其中一些药物诱导出了抗性；从每个菌株中分离出了克隆株。得出了这些克隆株对双脒、喹吖因（安锥赛）、吖啶黄素、菲啶类化合物以及其他对锥虫和利什曼原虫有活性的药物的交叉抗性模式。所测试的克隆株包括对吖啶黄素、安锥赛、二脒那秦（贝尼尔）和喷他脒有抗性的各两个克隆株以及对司替巴脒有抗性的一个克隆株。所有克隆株都明显存在相当程度的交叉抗性。在来自同一亲本菌株的克隆株之间观察到了差异。在大多数克隆株衍生群体中检测到了对异美汀和氧苯胂的协同敏感性。在无药物传代以检测抗药性的克隆株中，吖啶黄素和喷他脒克隆株在10次传代内失去抗性，而贝尼尔和安锥赛克隆株在无药物的情况下经过20至30次传代后仍保留相当程度的抗性。对生命周期差异的考虑表明，该克隆株集合可能有助于筛选对利什曼原虫和粪源性锥虫有效的药物，而不是对涎源性锥虫有效的药物。
