Chitambo H, Arakawa A
Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Japan.
Zentralbl Bakteriol. 1992 Oct;277(3):371-81. doi: 10.1016/s0934-8840(11)80916-3.
Four Trypanosoma congolense clones derived from a Mumbwa field isolate proved to be resistant to Berenil with a minimum curative dose (MCD) value of 40 mg/kg and to Samorin with an MCD of 4 mg/kg for mice. Two other clones, one being resistant to Berenil with an MCD of 45 mg/kg but susceptible to 1 mg/kg Samorin, and the other being resistant to Samorin with MCD of 16 mg/kg but susceptible to 7 mg/kg Berenil, were experimentally rendered resistant to each of the respective drugs they were susceptible to by subcurative treatments in mice. The original trypanosome strains and their derivative clones were then screened for their sensitivity to Berenil or Samorin. Three clones derived from the Mumbwa isolate were resistant to Berenil, with MCD's of 14 to 28 mg/kg, and to Samorin, with MCD's of 4 mg/kg. A single Mumbwa derivative clone was relatively sensitive to both Berenil with an MCD of 7 mg/kg and to Samorin with an MCD of 2 mg/kg. The reciprocal drug induction results confirmed that although trypanosomes can acquire tolerance to both Berenil and Samorin, no cross-resistance between the two was evident.
从姆布瓦野外分离株获得的四个刚果锥虫克隆对贝尼尔具有抗性，小鼠的最小治愈剂量（MCD）值为40毫克/千克，对沙莫林具有抗性，小鼠的MCD为4毫克/千克。另外两个克隆，一个对贝尼尔具有抗性，MCD为45毫克/千克，但对1毫克/千克沙莫林敏感，另一个对沙莫林具有抗性，MCD为16毫克/千克，但对7毫克/千克贝尼尔敏感，通过在小鼠中进行亚治愈剂量处理，使它们对各自敏感的药物产生抗性。然后对原始锥虫菌株及其衍生克隆进行贝尼尔或沙莫林敏感性筛选。从姆布瓦分离株获得的三个克隆对贝尼尔具有抗性，MCD为14至28毫克/千克，对沙莫林具有抗性，MCD为4毫克/千克。一个姆布瓦衍生克隆对贝尼尔相对敏感，MCD为7毫克/千克，对沙莫林相对敏感，MCD为2毫克/千克。相互药物诱导结果证实，尽管锥虫可以对贝尼尔和沙莫林都产生耐受性，但两者之间没有明显的交叉抗性。
