Delmas Delmas, Passilly-Degrace Patricia, Jannin Brigitte, Cherkaoui Malki Mustapha, Latruffe Norbert
Université de Bourgogne, LBMC, Faculté des Sciences, 6 Bd Gabriel, 21000 Dijon, France.
Int J Mol Med. 2002 Aug;10(2):193-9.
Resveratrol is a natural polyphenolic compound produced by a number of plants and found in high amount in peanuts, seeds, grapes or berries as source of human nutrition. Epidemiological studies strongly suggest that resveratrol may act as a cancer chemopreventive compound. The mechanism by which resveratrol inhibits cell proliferation was studied in human colorectal tumor SW480 cell line. The results show that resveratrol strongly inhibits cell proliferation at the micromolar range in a time- and dose-dependent manner. Resveratrol appears to block the cell cycle at the transition --> G2/M since inhibition of [(3)H]-thymidine incorporation is not observed, while there is an increase of the cell number in S phase. During this inhibition process, resveratrol increases the content of cyclins A and B1 as well as cyclin-dependent kinases Cdk1 and Cdk2. Moreover, resveratrol promotes Cdk1 phosphorylation. In conclusion, resveratrol exerts a strong inhibition of SW480 human colorectal tumor cell proliferation at least by modulating cyclin and cyclin-dependent kinase activities.
白藜芦醇是一种由多种植物产生的天然多酚化合物，在花生、种子、葡萄或浆果等作为人类营养来源的食物中含量很高。流行病学研究有力地表明，白藜芦醇可能是一种癌症化学预防化合物。在人结肠肿瘤SW480细胞系中研究了白藜芦醇抑制细胞增殖的机制。结果表明，白藜芦醇在微摩尔范围内以时间和剂量依赖性方式强烈抑制细胞增殖。白藜芦醇似乎在细胞周期从G1期到G2/M期的转变过程中阻止细胞周期，因为未观察到对[³H] - 胸腺嘧啶核苷掺入的抑制，而S期细胞数量增加。在这种抑制过程中，白藜芦醇增加了细胞周期蛋白A和B1以及细胞周期蛋白依赖性激酶Cdk1和Cdk2的含量。此外，白藜芦醇促进Cdk1磷酸化。总之，白藜芦醇至少通过调节细胞周期蛋白和细胞周期蛋白依赖性激酶的活性对SW480人结肠肿瘤细胞增殖产生强烈抑制作用。
