[一种用气相色谱法测定血液和尿液中尼古丁和可替宁的微量方法。来自各类吸烟者的结果]
[A micromethod for determination of nicotine and cotinine in blood and urine by gas chromatography. Results obtained from various smokers].
作者信息
Dumas C, Badré R, Viala A, Cano J P, Guillerm R
出版信息
Eur J Toxicol Environ Hyg. 1975 Sep-Oct;8(5):280-6.
The determination of nicotine and cotinine in plasma and urines of smokers is carried out by gas-chromatography with an Ucon-Polar 50 HB 2000 alkalinized column and a specific N detector, using diphenylamine as internal standard. The technique for extraction and chromatographic analysis is developed. The limit values for a correct determination are : 0,2 ng for nicotine and 1 ng for cotinine. The results show that the transit of nicotine and cotinine into the blood is very fast. They confirm the rapidity of the urinary excretion of nicotine, the slowness of that of cotinine, and the relation between the eliminated amounts and the urinary flow.
采用配备Ucon-Polar 50 HB 2000碱化柱和特定氮检测器的气相色谱法，以二苯胺作为内标，对吸烟者血浆和尿液中的尼古丁和可替宁进行测定。开发了萃取和色谱分析技术。正确测定的限值为：尼古丁0.2纳克，可替宁1纳克。结果表明，尼古丁和可替宁进入血液的过程非常迅速。这些结果证实了尼古丁经尿液排泄迅速，可替宁排泄缓慢，以及排泄量与尿流量之间的关系。
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