Feyerabend C, Russell M A
Nicotine Laboratory, New Cross Hospital, London, UK.
J Pharm Pharmacol. 1990 Jun;42(6):450-2. doi: 10.1111/j.2042-7158.1990.tb06592.x.
A rapid method is described for the simultaneous measurement of nicotine and cotinine in biological fluids using capillary column gas-liquid chromatography. Using 100 microliters sample volume the lower limit of detection for both nicotine and cotinine was 100 pg mL-1, allowing the method to be used for the measurement of these compounds in both smokers and non-smokers. The extraction time is 3 min per sample, and by using multi-pipetting and vortexing systems 250 samples can be extracted per day. The average coefficient of variation over the nicotine range 1.0 to 100 ng mL-1 was 3.9% and for cotinine over the range 1.0 to 1000 ng mL-1 was 2.2%. Saliva cotinine concentrations were quantitatively related to passive exposure to parental smoking in a population study of 1118 non-smoking schoolchildren.
描述了一种使用毛细管柱气液色谱法同时测量生物体液中尼古丁和可替宁的快速方法。使用100微升的样品体积，尼古丁和可替宁的检测下限均为100 pg mL-1，使得该方法可用于测量吸烟者和非吸烟者体内的这些化合物。每个样品的提取时间为3分钟，通过使用多管移液器和涡旋系统，每天可提取250个样品。在1.0至100 ng mL-1的尼古丁范围内，平均变异系数为3.9%，在1.0至1000 ng mL-1的可替宁范围内，平均变异系数为2.2%。在一项对1118名非吸烟学童的人群研究中，唾液可替宁浓度与被动接触父母吸烟存在定量关系。
