Kogan M J, Verebey K, Jaffee J H, Mulé S J
J Forensic Sci. 1981 Jan;26(1):6-11.
Human plasma levels of nicotine and its principal metabolite, cotinine, were simultaneously quantitated by gas-liquid chromatography combined with nitrogen selective detection. Nicotine, cotinine, and the added internal standard ketamine are extracted from plasma at basic pH into methylene chloride, back-extracted into acid, and then re-extracted into methylene chloride. Analysis is carried out on a packed glass column of 3% SE-30 while column temperature is programmed from 150 to 200 degrees C. Detector response is linea over the range of 2 to 50 ng/mL nicotine and 50 to 500 ng/mL cotinine. The method was validated on 150 plasma samples obtained from habitual smokers. Mean levels of 19.5 and 219 ng/mL were found for nicotine and cotinine, respectively. Both the mean and the range of the levels were in agreement with previously reported plasma levels for nicotine and cotinine.
采用气液色谱结合氮选择性检测法同时定量测定人血浆中尼古丁及其主要代谢物可替宁的含量。尼古丁、可替宁以及添加的内标物氯胺酮在碱性pH条件下从血浆中萃取至二氯甲烷中，再反萃取至酸性溶液中，然后重新萃取至二氯甲烷中。分析在填充有3% SE - 30的玻璃柱上进行，柱温从150℃程序升温至200℃。在2至50 ng/mL尼古丁和50至500 ng/mL可替宁范围内，检测器响应呈线性。该方法在从习惯性吸烟者获取的150份血浆样本上进行了验证。尼古丁和可替宁的平均含量分别为19.5 ng/mL和219 ng/mL。含量的平均值和范围均与先前报道的尼古丁和可替宁血浆水平一致。
