Jeong Jae Hoon, Kim Si Wouk, Yoon Seong Myeong, Park Jong Kun, Lee Jung Sup
Department of Biological Sciences, Chosun University, Gwangju, Korea.
Mol Cells. 2002 Jun 30;13(3):369-76.
The highly conserved region of the mxaF gene that encodes the large subunit of methanol dehydrogenase (MDH) was cloned and sequenced from Methylophaga sp. strain MP cells. The calculated G + C content of the conserved region was found to be 44.9%. The nucleotide sequence homology of the region to those from methylotrophs was approximately 43.5%, while the identity of the deduced amino acid sequence to other MxaF peptides was approximately 76.8%. Analysis of the codon usage revealed that UUC and CGU codons seem to be used only for phenylalanine and arginine, respectively. The aligned amino acid sequences show that several key amino acids that are required for the MDH enzyme activity are located in the deduced MxaF peptide, together with tryptophan-docking motifs, called W4 and W5.
从食甲基菌属菌株MP细胞中克隆并测序了编码甲醇脱氢酶（MDH）大亚基的mxaF基因的高度保守区域。发现该保守区域的计算G + C含量为44.9%。该区域与甲基营养菌的核苷酸序列同源性约为43.5%，而推导的氨基酸序列与其他MxaF肽的同一性约为76.8%。密码子使用情况分析表明，UUC和CGU密码子似乎分别仅用于苯丙氨酸和精氨酸。比对的氨基酸序列显示，MDH酶活性所需的几个关键氨基酸位于推导的MxaF肽中，还有称为W4和W5的色氨酸对接基序。
