Vorob'ev Alexey V, de Boer Wietse, Folman Larissa B, Bodelier Paul L E, Doronina Nina V, Suzina Natalia E, Trotsenko Yuri A, Dedysh Svetlana N
S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2538-45. doi: 10.1099/ijs.0.010074-0. Epub 2009 Jul 21.
Two strains of Gram-negative, aerobic, non-pigmented, non-motile, rod-shaped bacteria were isolated from beechwood blocks during decay by the white-rot fungus Hypholoma fasciculare and were designated strains BW863(T) and BW872. They are capable of methylotrophic growth and assimilate carbon via the ribulose-bisphosphate pathway. In addition to methanol, the novel isolates utilized ethanol, pyruvate and malate. Strains BW863(T) and BW872 are obligately acidophilic, mesophilic organisms capable of growth at pH 3.1-6.5 (with an optimum at pH 4.5-5.0) and at 4-30 degrees C. Phospholipid fatty acid profiles of these bacteria contain unusually large amounts (about 90 %) of C(18 : 1)omega7c, thereby resembling the profiles of Methylobacterium strains. The predominant quinone is Q-10. The DNA G+C content of the novel isolates is 61.8-62.8 mol%. On the basis of 16S rRNA gene sequence similarity, strains BW863(T) and BW872 are most closely related to the acidophilic methanotroph Methylocapsa acidiphila B2(T) (96.5-97 %). Comparative sequence analysis of mxaF, the gene encoding the large subunit of methanol dehydrogenase, placed the MxaF sequences of the two novel strains in a cluster that is distinct from all previously described MxaF sequences of cultivated methylotrophs. The identity between the MxaF sequences of the acidophilic isolates and those from known alpha-, beta- and gammaproteobacterial methylotrophs was respectively 69-75, 61-63 and 64-67 %. The data therefore suggest that strains BW863(T) and BW872 represent a novel genus and species of methylotrophic bacteria, for which the name Methylovirgula ligni gen. nov., sp. nov. is proposed. Strain BW863(T) (=DSM 19998(T) =NCIMB 14408(T)) is the type strain of Methylovirgula ligni.
从被白腐菌簇生丝盖伞（Hypholoma fasciculare）分解的山毛榉木块中分离出两株革兰氏阴性、需氧、无色素、不运动的杆状细菌，分别命名为BW863(T)和BW872菌株。它们能够进行甲基营养生长，并通过核酮糖-二磷酸途径同化碳。除甲醇外，这些新分离株还利用乙醇、丙酮酸和苹果酸。BW863(T)和BW872菌株是专性嗜酸、嗜温菌，能在pH 3.1 - 6.5（最适pH 4.5 - 5.0）和4 - 30℃下生长。这些细菌的磷脂脂肪酸谱含有异常大量（约90%）的C(18 : 1)ω7c，因此类似于甲基杆菌属菌株的谱。主要醌为Q - 10。新分离株的DNA G + C含量为61.8 - 62.8 mol%。基于16S rRNA基因序列相似性，BW863(T)和BW872菌株与嗜酸甲烷氧化菌嗜酸甲基帽菌（Methylocapsa acidiphila）B2(T)关系最为密切（96.5 - 97%）。对编码甲醇脱氢酶大亚基的基因mxaF进行比较序列分析，将这两个新菌株的MxaF序列置于一个与所有先前描述的培养甲基营养菌的MxaF序列不同的簇中。嗜酸分离株与已知的α -、β -和γ -变形杆菌甲基营养菌的MxaF序列同一性分别为69 - 75%、61 - 63%和64 - 67%。因此，数据表明BW863(T)和BW872菌株代表一种新的甲基营养细菌属和种，为此提议命名为嗜木甲基微菌属（Methylovirgula ligni），新种。BW863(T)菌株（=DSM 19998(T)=NCIMB 14408(T)）是嗜木甲基微菌的模式菌株。
