Hafter R, Schneebauer T, Tafel K, Ernst E, Graeff H
Geburtshilfe Frauenheilkd. 1975 Jul;35(7):518-25.
The quantitative estimation of soluble fibrin monomer complexes (SFMC) was applied to evaluate the state of hypercoagulability during pregnancy and delivery. Blood samples from 67 healthy primi and multiparae, 6 to 40 weeks pregnant, and from a group of 8 women in labour and after delivery of the placenta were examined. Fibrinogen and SFMC were precipitated from plasma by precipitation with beta-alanine. Gel filtration (4% agarose) of the redissolved precipitate resulted in a separation of SFMC and fibrinogen. This enabled a quantitative estimation of the SFMC concentration (with-in assay precision: coefficient of variation=8%). The % amount SFMC of the total fibrinogen content increased from 2.6 +/- 0.4 to 4.9 +/- 1.3% (mean and standard deviation) to week 40 of pregnancy. During delivery an additional statistically significant increase occurred. Chain analysis of SFMC showed a decreased amount of alpha-chain indicating plasmin activity. gamma-gamma-dimers as residuals of intermolecular covalent bonding were not observed. The quantitative estimation of SFMC during pregnancy and delivery demonstrates that a state of hypercoagulability during gestation can be evaluated by measuring the catabolic products of fibrinogen. This may lead to a differentiation from severe intravascular coagulation and to an early diagnosis of thromboembolic disease or consumption coagulopathy.
应用可溶性纤维蛋白单体复合物（SFMC）的定量测定来评估妊娠和分娩期间的高凝状态。对67例妊娠6至40周的健康初产妇和经产妇以及一组8例分娩时及胎盘娩出后的妇女的血样进行了检测。通过用β-丙氨酸沉淀从血浆中沉淀出纤维蛋白原和SFMC。对重新溶解的沉淀物进行凝胶过滤（4%琼脂糖）可分离出SFMC和纤维蛋白原。这使得能够对SFMC浓度进行定量测定（测定内精密度：变异系数=8%）。妊娠40周时，SFMC占总纤维蛋白原含量的百分比从2.6±0.4增加到4.9±1.3%（均值和标准差）。分娩期间出现了另外的统计学显著增加。SFMC的链分析显示α链数量减少，表明有纤溶酶活性。未观察到作为分子间共价键残余物的γ-γ二聚体。妊娠和分娩期间对SFMC的定量测定表明，通过测量纤维蛋白原的分解产物可以评估妊娠期的高凝状态。这可能有助于与严重血管内凝血相鉴别，并早期诊断血栓栓塞性疾病或消耗性凝血病。
