Sun Xuguang, Wang Zhiqun, Luo Shiyun, Jin Xiuying, Zhang Wenhua
Capital University of Medical Sciences, Beijing Tong Ren Ophthalmic Center, Beijing 100730, China.
Zhonghua Yan Ke Za Zhi. 2002 May;38(5):292-4.
To study the distribution and shifting trends of corneal bacterial isolates from bacterial keratitis retrospectively.
The data of bacterial cultures of 2 220 corneal isolates from January, 1989 to December, 1998 were reviewed.
During this 10-year period, 2 220 consecutive corneal cultures were obtained, and a positive-culture was recovered in 490 isolates, the positive rate being 22.1%. In the 490 positive-cultures, Gram-positive cocci and Gram-negative bacilli represented 51.0% and 39.4% respectively. Gram-positive bacilli was 9.2%. Pseudomonas aeruginosa was isolated the most commonly in the corneal isolates (32.2%), followed by coagulase-negative staphylococcus (18.6%). Streptococcus pneumoniae represented 12.0%. During this period, we documented a gradual increase in the number of Gram-positive cocci coupled with a decrease of the number of Gram-negative bacilli.
Pseudomonas aeruginosa and coagulase-negative staphylococcus are the most common pathogens in bacterial keratitis in the north part of China. At present, the increased recovery of Gram-positive cocci and decreased of Gram-negative bacilli from keratitis isolates present an important challenge to the ophthalmology.
回顾性研究细菌性角膜炎角膜细菌分离株的分布及变化趋势。
回顾1989年1月至1998年12月2220例角膜分离株的细菌培养数据。
在这10年期间，共获得2220例连续的角膜培养物，490例分离株培养结果为阳性，阳性率为22.1%。在490例阳性培养物中，革兰氏阳性球菌和革兰氏阴性杆菌分别占51.0%和39.4%。革兰氏阳性杆菌占9.2%。铜绿假单胞菌是角膜分离株中最常见的分离菌（32.2%），其次是凝固酶阴性葡萄球菌（18.6%）。肺炎链球菌占12.0%。在此期间，我们记录到革兰氏阳性球菌数量逐渐增加，革兰氏阴性杆菌数量减少。
铜绿假单胞菌和凝固酶阴性葡萄球菌是中国北方细菌性角膜炎最常见的病原体。目前，角膜炎分离株中革兰氏阳性球菌回收率增加而革兰氏阴性杆菌减少，这给眼科带来了重要挑战。
