Kim Kyung Jin, Howard Andrew J
Industrial Macromolecular Crystallography Association Access Team (IMCA-CAT), Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA.
Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1332-4. doi: 10.1107/s0907444902007953. Epub 2002 Jul 20.
Saccharomyces cerevisiae alcohol dehydrogenase I crystallized as trigonal plates using 20% 2-propanol and 20% PEG 4000 in 0.1 M sodium citrate buffer pH 5.6 in the presence of 1 mM NAD(+). The crystals diffract to 3.0 A resolution and belong to the trigonal space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 146.3, c = 68.1 A, alpha = beta = 90, gamma = 120 degrees. X-ray data were collected from frozen crystals at the 17-ID beamline of the Advanced Photon Source. A Zn fluorescence scan of the crystal produced a peak at 9671.6 eV, suggesting the existence of Zn ions in the crystal.
