Crystallization and preliminary X-ray crystallographic studies of rice alpha-galactosidase.
作者信息
Fujimoto Zui, Kobayashi Osamu, Kaneko Satoshi, Momma Mitsuru, Kobayashi Hideyuki, Mizuno Hiroshi
机构信息
Department of Biochemistry, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan.
出版信息
Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1374-5. doi: 10.1107/s0907444902010168. Epub 2002 Jul 20.
alpha-Galactosidases catalyze the hydrolysis of galactooligosaccharides and galactopolysaccharides to alpha-galactose residues and are widely distributed in microorganisms, plants and animals. alpha-Galactosidase from rice (Oryza sativa L. ssp. japonica) was crystallized by the hanging-drop vapour-diffusion method. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 63.1, b = 71.3, c = 85.6 A, and diffract beyond 1.9 A resolution.
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