Huang Jen-Yen, Chang Chia-Yu, Chang Tschining, Chen Chun-Jung
X-ray Structural Biology Group, National Synchrotron Radiation Research Center, Hsinchu 30077, Taiwan, and Department of Life Sciences, National Tsing-Hua University, Hsinchu 30077, Taiwan.
Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1648-50. doi: 10.1107/s0907444903014616. Epub 2003 Aug 19.
Nucleoside diphosphate kinase (NDK) catalyses the transfer of the gamma-phosphoryl group from a nucleoside triphosphate (NTP) to a nucleoside diphosphate (NDP). NDK is involved in and required for coleoptile elongation in rice. The level of the enzyme changes during seed germination and the early stages of seedling growth. Recombinant rice NDK (rNDK) has been crystallized using the hanging-drop vapour-diffusion method. rNDK crystals diffracted to a resolution of 2.50 A and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.98, b = 182.26, c = 188.30 A. Preliminary analysis indicates there to be 12 rNDK molecules in each asymmetric unit, with a solvent content of 47.2%.
核苷二磷酸激酶（NDK）催化γ-磷酸基团从核苷三磷酸（NTP）转移至核苷二磷酸（NDP）。NDK参与水稻胚芽鞘伸长过程并发挥作用。在种子萌发和幼苗生长早期，该酶的水平会发生变化。已采用悬滴气相扩散法使重组水稻NDK（rNDK）结晶。rNDK晶体的衍射分辨率为2.50 Å，属于空间群P2(1)2(1)2(1)，晶胞参数a = 70.98、b = 182.26、c = 188.30 Å。初步分析表明，每个不对称单元中有12个rNDK分子，溶剂含量为47.2%。
