Fujimoto Zui, Kim Wook-Dong, Kaneko Satoshi, Park Gwi-Gun, Momma Mitsuru, Kobayashi Hideyuki, Mizuno Hiroshi
Department of Biochemistry, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan.
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2289-91. doi: 10.1107/s0907444903019681. Epub 2003 Nov 27.
alpha-Galactosidases catalyze the hydrolysis of a galactosyl residue from galactooligosaccharides and galactopolysaccharides. alpha-Galactosidase I from Mortierella vinacea was crystallized in two crystal forms using the hanging-drop vapour-diffusion method. Type 1 crystals belonged to space group I422, with unit-cell parameters a = b = 142.4, c = 131.5 A, and diffracted to beyond 2.1 A resolution, while type 2 crystals belonged to space group P4, with unit-cell parameters a = b = 100.9, c = 102.7 A, and diffracted to beyond 1.6 A resolution. This enzyme crystallized as a glycoprotein tetramer and the tetrameric structure was located around the crystallographic fourfold axis.
α-半乳糖苷酶催化从低聚半乳糖和半乳聚糖中水解出一个半乳糖基残基。使用悬滴气相扩散法，将来自酒红被孢霉的α-半乳糖苷酶I结晶为两种晶体形式。1型晶体属于空间群I422，晶胞参数a = b = 142.4，c = 131.5 Å，衍射分辨率超过2.1 Å；而2型晶体属于空间群P4，晶胞参数a = b = 100.9，c = 102.7 Å，衍射分辨率超过1.6 Å。这种酶结晶为糖蛋白四聚体，四聚体结构位于晶体学四重轴周围。
