Xu Qiang, Wang Ke-Yi
Shanghai Institute of Biochemistry the Chinese Academy of Sciences Shanghai 200031 China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31(2):111-115.
Glycosylation the addition of sugar residues to peptide backbone is the most common post-translational modification of proteins in eukaryotic cells and has important functions in secretion antigenicity and metabolism of glycoproteins. Majority of proteins of pharmaceutical interest are glycoproteins. In recent years great advances have been made in understanding the versatile biological functions of carbohydrates in the accurate analysis of glycoprotein heterogeneity and in controlling the biosynthetic pathways of glycosylation. The aim of this mini review is to discuss general advantages and potential problems of different systems for expressing recombinant glycoproteins.
糖基化，即将糖残基添加到肽主链上，是真核细胞中蛋白质最常见的翻译后修饰，并且在糖蛋白的分泌、抗原性和代谢方面具有重要功能。大多数具有药物价值的蛋白质都是糖蛋白。近年来，在理解碳水化合物的多种生物学功能、准确分析糖蛋白异质性以及控制糖基化生物合成途径方面取得了巨大进展。本综述的目的是讨论表达重组糖蛋白的不同系统的一般优势和潜在问题。
