Zamir Ashira, Shpitzen Moshe, Oz Carla, Motro Uzi, Meiner Vardiela, Gafny Ron
Forensic Biology Laboratory, Division of Identification and Forensic Science, Israel Police, National HQ, Jerusalem.
J Forensic Sci. 2002 Jul;47(4):824-6.
The Israel police forensic biology laboratory received as an item of evidence in an attempted murder case, a pair of trousers belonging to a suspect. A bloodstain was observed on the trousers and analyzed by STR typing for nine loci using the Promega GenePrint STR silver stain detection kits. The genetic profile defined was found to be identical to that of the victim's at all nine loci. Within this profile a three-banded allele pattern was observed at the D16S539 locus, both in the bloodstain and in the victim's reference blood sample. Confirmation of this phenomenon was accomplished by amplifying the extracted DNA from both the trousers and the victim's blood sample using the PowerPlex 16 kit by Promega and the AmpFlSTR SGM Plus kit by Perkin Elmer, followed by analysis of the amplification products by capillary electrophoresis on the ABI prism 310 genetic analyzer. The same three-banded allele pattern was observed at the D16S539 locus in both specimen and reference DNA, using each of the three kits. Three additional loci located on chromosome 16 (D16S3407, D16S2617 and D16S3082), not employed for forensic identification, were also analyzed and did not show three-banded allele pattern.
以色列警方法医生物学实验室在一桩谋杀未遂案件中收到了一件证据，即嫌疑人的一条裤子。在这条裤子上发现了一处血迹，并使用Promega GenePrint STR银染检测试剂盒对九个基因座进行STR分型分析。结果发现，所确定的基因图谱在所有九个基因座上都与受害者的基因图谱相同。在这个基因图谱中，在D16S539基因座上，血迹和受害者的参考血样中均观察到一种三条带的等位基因模式。通过使用Promega公司的PowerPlex 16试剂盒和Perkin Elmer公司的AmpFlSTR SGM Plus试剂盒对从裤子和受害者血样中提取的DNA进行扩增，然后在ABI prism 310基因分析仪上通过毛细管电泳对扩增产物进行分析，证实了这一现象。使用这三种试剂盒中的每一种，在样本DNA和参考DNA的D16S539基因座上均观察到相同的三条带等位基因模式。还对位于16号染色体上的另外三个未用于法医鉴定的基因座（D16S3407、D16S2617和D16S3082）进行了分析，未发现三条带等位基因模式。
