Gorbushina A A, Lialikova N N, Vlasov D Iu, Khizhniak T V
Laboratory of Lower Plants, Biological Research Institute, Stary Peterhof, St. Petersburg, Russia.
Mikrobiologiia. 2002 May-Jun;71(3):409-17.
Stone monuments situated in the Alexander Nevsky Abbey, the Summer Garden, and the Smolenskoe Cemetery, St. Petersburg, and marble and limestone sculptures and tombstones situated in the Novodevichy Convent and the Donskoy Monastery, Moscow, were investigated for their microbial contamination. The architectural objects studied date to the 12th century. The monuments in the Alexander Nevsky Abbey were found to be severely contaminated with micromycetes belonging to 24 genera (primarily of the class Deuteromycetes). The analysis of the samples taken from the monuments of the Donskoy Monastery by the serial dilution technique showed that they were contaminated with bacteria at a density of (1-1.7) x 10(5) cells/g. This value, however, turned out to be 1 to 2 orders greater when the bacterial population was evaluated by the luciferin-luciferase method. We succeeded in identifying 12 bacterial genera; however, this number may be increased in the course of further studies. Some preventive measures to control the biodeterioration of stone heritage are discussed.
对位于圣彼得堡亚历山大·涅夫斯基修道院、夏园和斯莫尔尼斯科耶公墓的石碑,以及位于莫斯科新圣女修道院和顿斯科伊修道院的大理石和石灰石雕塑及墓碑进行了微生物污染调查。所研究的建筑古迹可追溯到12世纪。发现亚历山大·涅夫斯基修道院内的古迹受到严重污染,有属于24个属的微真菌(主要是半知菌类)。通过连续稀释技术对从顿斯科伊修道院古迹采集的样本进行分析表明,它们被细菌污染,密度为(1 - 1.7)×10(5)个细胞/克。然而,当用荧光素 - 荧光素酶法评估细菌数量时,这个值高出1至2个数量级。我们成功鉴定出12个细菌属;不过,在进一步研究过程中这个数量可能会增加。还讨论了一些控制石材遗产生物劣化的预防措施。