幽门螺杆菌组氨酸激酶突变体的全基因组转录谱分析确定了一个调控子的成员。
Genome-wide transcriptional profiling in a histidine kinase mutant of Helicobacter pylori identifies members of a regulon.
作者信息
Forsyth Mark H, Cao Ping, Garcia Preston P, Hall Joshua D, Cover Timothy L
机构信息
Department of Biology, The College of William and Mary, Williamsburg, Virginia 23187, USA.
出版信息
J Bacteriol. 2002 Aug;184(16):4630-5. doi: 10.1128/JB.184.16.4630-4635.2002.
Abstract
To identify putative members of a regulon controlled by the H. pylori sensory histidine kinase HP0164 (HK0164), we constructed HK0164 null mutant H. pylori strains and analyzed bacterial gene transcription using DNA arrays. Seven genes were differentially expressed in multiple HK0164 mutant strains compared to their expression in control strains. Strain-dependent variations in differential expression were also detected. These results indicate that the signal transduction circuit utilizing HK0164 controls the transcription of at least seven genes in H. pylori.
摘要
为了鉴定由幽门螺杆菌传感组氨酸激酶HP0164（HK0164）控制的调节子的假定成员，我们构建了HK0164缺失突变的幽门螺杆菌菌株，并使用DNA阵列分析细菌基因转录。与对照菌株中的表达相比，7个基因在多个HK0164突变菌株中差异表达。还检测到差异表达中的菌株依赖性变异。这些结果表明，利用HK0164的信号转导回路控制幽门螺杆菌中至少7个基因的转录。
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