组织微阵列：免疫组织化学质量控制的新方法。
Tissue microarrays: a new approach for quality control in immunohistochemistry.
作者信息
Packeisen J, Buerger H, Krech R, Boecker W
机构信息
Department of Pathology, Klinikum Osnabrueck, Am Finkenhuegel 1, 49076 Osnabrueck, Germany.
出版信息
J Clin Pathol. 2002 Aug;55(8):613-5. doi: 10.1136/jcp.55.8.613.
AIMS
To improve the interpretation of immunohistochemistry (IHC) staining results the use of a tissue microarray technique was established in a routine setting.
METHODS
A tissue microarray was constructed by harvesting 600 microm tissue cores from paraffin wax embedded samples available in a routine pathology department. The punches originating from non-tumorous tissue were placed on host paraffin wax blocks. The microarray contained 12 different tissue samples, with a wide antigen profile and a dimension of 3.5 x 3 mm. One section of the multitissue array was placed as an "internal" positive control on each slide of the patient tissue to undergo identical immunohistochemical procedures.
RESULTS
Using the tissue microarray technique as a tool for internal quality control, the interpretation of immunohistochemical staining of more than 20 different antigens in routine IHC was improved. The tissue microarray did not influence the staining results in conventional IHC or in different automated IHC settings.
CONCLUSION
The regular use of an institution adapted tissue microarray would be useful for internal positive control in IHC to enable different laboratory demands. Furthermore, this technique improves the evaluation of staining results in IHC.
目的
为了改进免疫组织化学（IHC）染色结果的解读，在常规环境中建立了组织微阵列技术的应用。
方法
通过从常规病理科可用的石蜡包埋样本中获取600微米的组织芯构建组织微阵列。来自非肿瘤组织的打孔样本被放置在宿主石蜡块上。该微阵列包含12种不同的组织样本，具有广泛的抗原谱，尺寸为3.5×3毫米。多组织阵列的一个切片作为“内部”阳性对照放置在每个患者组织切片上，以进行相同的免疫组织化学程序。
结果
使用组织微阵列技术作为内部质量控制工具，常规免疫组化中20多种不同抗原的免疫组化染色解读得到了改进。组织微阵列不影响传统免疫组化或不同自动化免疫组化设置中的染色结果。
结论
定期使用机构定制的组织微阵列对于免疫组化中的内部阳性对照以满足不同实验室需求是有用的。此外，该技术改进了免疫组化染色结果的评估。
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