开发一种优化的、简单的化学成分确定的培养基，用于醋杆菌属A9在摇瓶培养中生产细菌纤维素。
Development of an optimized, simple chemically defined medium for bacterial cellulose production by Acetobacter sp. A9 in shaking cultures.
作者信息
Heo Moon-Soo, Son Hong-Joo
机构信息
Faculty of Applied Marine Science, Cheju National University, Jeju 690-756, South Korea.
出版信息
Biotechnol Appl Biochem. 2002 Aug;36(1):41-5. doi: 10.1042/ba20020018.
The genus Acetobacter can synthesize cellulose when grown in an undefined medium containing glucose. By using the technique of the omission of a single medium component, an optimized and simple chemically defined medium was developed to support cellulose production by Acetobacter sp. A9 in shaking culture. It contained 4.0% (w/v) glucose, 0.2% (w/v) (NH(4))(2)SO(4), 0.25% (w/v) KH(2)PO(4), 0.3% (w/v) Na(2)HPO(4).12H(2)O, 0.05% (w/v) MgSO(4).7H(2)O, 0.0002% (w/v) FeSO(4).7H(2)O, 0.00025% (w/v) H(3)BO(3), 0.00006% (w/v) nicotinamide, 0.00025% (w/v) inositol and 1.4% (v/v) ethanol. A maximum cellulose concentration of around 8 g/l was achieved after 9 days of cultivation at 200 rev./min. The production of cellulose by Acetobacter sp. A9 was greater in simplified synthetic medium than in complex medium (Hestrin and Schramm medium) conventionally used for Acetobacter strains.
醋酸杆菌属在含有葡萄糖的成分不明的培养基中生长时能够合成纤维素。通过采用逐一去除单一培养基成分的技术，开发出了一种优化且简单的化学成分明确的培养基，以支持醋酸杆菌A9在摇瓶培养中生产纤维素。该培养基含有4.0%（w/v）葡萄糖、0.2%（w/v）硫酸铵、0.25%（w/v）磷酸二氢钾、0.3%（w/v）十二水磷酸氢二钠、0.05%（w/v）七水硫酸镁、0.0002%（w/v）七水硫酸亚铁、0.00025%（w/v）硼酸、0.00006%（w/v）烟酰胺、0.00025%（w/v）肌醇和1.4%（v/v）乙醇。在200转/分钟的转速下培养9天后，纤维素的最大浓度达到约8克/升。醋酸杆菌A9在简化合成培养基中的纤维素产量高于传统用于醋酸杆菌菌株的复合培养基（赫斯特林和施拉姆培养基）。
Zotero 插件