[Determination of optimal conditions for physical and chemical inactivation of nuclei for obtaining haploid and anuclear loach (Misgurnus fossilis L.) embryos].

The doses of X-irradiation sufficient for obtained truly haploid and anuclear embryos of the loach were determined. Mitomycin C in concentrations arresting the development at the late blastula stage (100-500 mcg/ml) was tested for the purposes of chemical enucleation of embryos. The data obtained allowed to recommend the following doses of X-irradiation to obtained the 100% inactivation of one or both the paternal genomes and haploid and anuclear embryos of the loach: 40 kr for eggs and 60 kr for testes. Mitomycin in the concentrations tested did not cause the 100% enucleation of embryos.