配备微透镜和尼普科夫圆盘的高速1帧/毫秒扫描共聚焦显微镜。

High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks.

作者信息

Tanaami Takeo, Otsuki Shinya, Tomosada Nobuhiro, Kosugi Yasuhito, Shimizu Mizuho, Ishida Hideyuki

机构信息

Yokogawa Electric Corporation, Tokyo, Japan.

出版信息

Appl Opt. 2002 Aug 1;41(22):4704-8. doi: 10.1364/ao.41.004704.

DOI:10.1364/ao.41.004704

PMID:12153106

Abstract

We have developed a high-speed confocal laser microscope. A microlens-array disk set in front of a pinhole-array disk improved optical efficiency more than ten times compared with that of conventional Nipkow confocal microscopy. This new microscope achieves a high-speed measurement of 1 frame/ms. We expect that it will be used for measuring biological and industrial active samples.

摘要

我们开发了一种高速共聚焦激光显微镜。与传统的尼普科夫共聚焦显微镜相比，置于针孔阵列盘前方的微透镜阵列盘将光学效率提高了十多倍。这种新型显微镜实现了1帧/毫秒的高速测量。我们期望它将用于测量生物和工业活性样品。