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微机械透射扫描共聚焦显微镜

Micromachined transmissive scanning confocal microscope.

作者信息

Kwon Sunghoon, Lee Luke P

机构信息

Berkeley Sensor and Actuator Center, Department of Bioengineering, University of California, Berkeley, California 94720, USA.

出版信息

Opt Lett. 2004 Apr 1;29(7):706-8. doi: 10.1364/ol.29.000706.

Abstract

We have developed a miniature scanning confocal microscope that uses electrostatically actuated microlenses for focusing and scanning. Objective lenses, scanners, a pupil, and a pinhole of the confocal microscope are microfabricated and integrated into a volume smaller than 2 mm3 by stacking these components. Objective lenses are composed of two vertically cascaded polymer microlenses integrated into micromachined comb actuators. Raster scanning is implemented by electrostatically actuating each microlens in orthogonal directions. We have demonstrated reflection confocal imaging with 3-microm transverse resolution over a 100-microm field of view and a 0.38-mm working distance at lambda = 633 nm.

摘要

我们开发了一种微型扫描共聚焦显微镜,它使用静电驱动的微透镜进行聚焦和扫描。共聚焦显微镜的物镜、扫描器、光瞳和针孔通过堆叠这些组件进行微加工并集成到小于2立方毫米的体积中。物镜由两个垂直级联的聚合物微透镜组成,集成到微机械梳状致动器中。通过在正交方向上静电驱动每个微透镜来实现光栅扫描。我们已经证明,在λ = 633 nm时,在100微米的视场和0.38毫米的工作距离上具有3微米的横向分辨率的反射共聚焦成像。

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