使用磁驱动共聚焦微型扫描器进行垂直光学切片，旨在用于体内临床成像。

Vertical optical sectioning using a magnetically driven confocal microscanner aimed for in vivo clinical imaging.

作者信息

Mansoor Hadi, Zeng Haishan, Chen Keqin, Yu Yingqiu, Zhao Jianhua, Chiao Mu

机构信息

Department of Mechanical Engineering, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.

出版信息

Opt Express. 2011 Dec 5;19(25):25161-72. doi: 10.1364/OE.19.025161.

DOI:10.1364/OE.19.025161

PMID:22273907

Abstract

This paper presents a confocal microscanner for direct vertical optical sectioning of biological samples. Confocal imaging is performed by transverse (X-axis) and axial (Z-axis) scanning of a focused laser beam using an optical fiber and a microlens respectively. The actuators are fabricated by laser micromachining techniques and are driven by electromagnetic forces. Optical and mechanical performance of the system is predicted by simulation software packages and characterized by experimental measurements. The scanner has lateral resolution of 3.87 µm and axial resolution of 10.68 µm with a field of view of 145 µm in X and 190 µm in Z directions. Confocal imaging of a polymer layer deposited on a silicon wafer and onion epidermal cells is demonstrated.

摘要

本文介绍了一种用于对生物样品进行直接垂直光学切片的共聚焦显微镜扫描器。共聚焦成像分别通过使用光纤和微透镜对聚焦激光束进行横向（X轴）和轴向（Z轴）扫描来实现。这些致动器采用激光微加工技术制造，并由电磁力驱动。该系统的光学和机械性能通过仿真软件包进行预测，并通过实验测量进行表征。该扫描器的横向分辨率为3.87 µm，轴向分辨率为10.68 µm，在X方向上的视野为145 µm，在Z方向上的视野为190 µm。文中展示了对沉积在硅片上的聚合物层和洋葱表皮细胞的共聚焦成像。