人类胎儿大脑中一种新型脱氢酶/还原酶（SDR家族）成员1基因的分子克隆与特性分析
Molecular cloning and characterization of a novel Dehydrogenase/reductase (SDR family) member 1 genea from human fetal brain.
作者信息
Wu Q, Xu M, Cheng C, Zhou Z, Huang Y, Zhao W, Zeng L, Xu J, Fu X, Ying K, Xie Y, Mao Y
机构信息
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, PR China.
出版信息
Mol Biol Rep. 2001;28(4):193-8. doi: 10.1023/a:1015722001960.
Short-chain dehydrogenases/reductases (SDR) constitute a large protein family of NAD(P)(H)-dependent oxidoreductase. They are defined by distinct, common sequence motifs and show a wide range of substrate specialisms. By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a novel human SDR-type dehydrogenase/reductase gene named Dehydrogenase/reductase (SDR family) member 1 (DHRS1). The DHRS1 cDNA is 1411 base pair in length, encoding a 314-amino-acid polypeptide which has a SDR motif. Northern blot reveals two bands, of about 0.9 and 1.4 kb in size. These two forms are expressed in many tissues. The DHRS1 gene is localized on chromosome 14q21.3. It has 9 exons and spans 9.2 kb of the genomic DNA.
短链脱氢酶/还原酶（SDR）构成了一个依赖NAD(P)(H)的氧化还原酶的大型蛋白质家族。它们由独特的共同序列基序定义，并表现出广泛的底物特异性。通过对人类胎儿脑cDNA文库的大规模测序分析，我们分离出一个新的人类SDR型脱氢酶/还原酶基因，命名为脱氢酶/还原酶（SDR家族）成员1（DHRS1）。DHRS1 cDNA长度为1411个碱基对，编码一个具有SDR基序的314个氨基酸的多肽。Northern印迹显示两条带，大小约为0.9和1.4 kb。这两种形式在许多组织中表达。DHRS1基因定位于14号染色体q21.3。它有9个外显子，跨越9.2 kb的基因组DNA。
Zotero 插件