从胎儿大脑中克隆并鉴定一种新型人类类羟类固醇脱氢酶2（HSDL2）cDNA
Molecular cloning and characterization of a novel human hydroxysteroid dehydrogenase-like 2 (HSDL2) cDNA from fetal brain.
作者信息
Dai Jianfeng, Xie Yi, Wu Qihan, Wang Liu, Yin Gang, Ye Xin, Zeng Li, Xu Jian, Ji Chaoneng, Gu Shaohua, Huang Qingshan, Zhao Robert Chunhua, Mao Yumin
机构信息
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
出版信息
Biochem Genet. 2003 Jun;41(5-6):165-74. doi: 10.1023/a:1023377627138.
Hydroxysteroid dehydrogenases (HSDs) are responsible for the biosynthesis of steroid hormones and play a crucial role in mammalian physiology and development. By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a novel human hydroxysteroid dehydrogenase-like cDNA (HSDL2). This cDNA is 3211 bp in length, encoding a 418-amino-acid polypeptide, which contains a typical motif for NAD(P)+-binding (TGxxxGxG), an SDR active site motif (S-Y-K) and a sterol carrier protein domain. HSDL2 shows high similarity with the homologues in the mouse and fruit fly. The HSDL2 gene is mapped to chromosome 9q32 and contains 11 exons. RT-PCR analysis shows that the HSDL2 gene is widely expressed in human tissues and the expression levels in liver, kidney, prostate, testis, and ovary are relatively high.
羟基类固醇脱氢酶（HSDs）负责甾体激素的生物合成，在哺乳动物的生理和发育过程中发挥着关键作用。通过对人类胎儿脑cDNA文库进行大规模测序分析，我们分离出了一种新的人类羟基类固醇脱氢酶样cDNA（HSDL2）。该cDNA长度为3211 bp，编码一个418个氨基酸的多肽，其中包含一个典型的NAD(P)+结合基序（TGxxxGxG）、一个短链脱氢酶/还原酶（SDR）活性位点基序（S-Y-K）和一个固醇载体蛋白结构域。HSDL2与小鼠和果蝇中的同源物具有高度相似性。HSDL2基因定位于9号染色体q32区域，包含11个外显子。逆转录-聚合酶链反应（RT-PCR）分析表明，HSDL2基因在人体组织中广泛表达，在肝脏、肾脏、前列腺、睾丸和卵巢中的表达水平相对较高。
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