Levine L, Alam I
Prostaglandins Med. 1979 Nov;3(5):295-304. doi: 10.1016/0161-4630(79)90071-5.
Biosynthesis of PGI2, measured as 6-keto-PGF1 alpha, thromboxane A2, measured as thromboxine B2, and prostaglandins E2, F2 alpha and D2 by lymphocytes (WEHI-5), endothelial cells, normal human lung cells (WI-38), normal human fibroblasts (D-550), rat adult Type II alveolar cells (L-2) and canine kidney cells (MDCK) was measured by radioimmunoassay of culture fluids before and after their separation by high pressure liquid chromatography. The metabolic profiles and the levels of each metabolite obtained by both procedures were comparable. The profile of arachidonic biosynthesis was unique to each cell. Endothelial cells synthesized primarily prostacyclin; the lymphocytes synthesized principally thromboxane. The dog kidney cells synthesized relatively large amounts of prostaglandin F2 alpha, I2 and E2, while the normal human lung cells produced predominantly prostaglandins E2, F2 alpha, and thromboxane. The rat adult alveolar cell (L-2) and the normal human fibroblasts (D-550) biosynthesized primarily prostaglandins E2 and F2 alpha.
通过对淋巴细胞（WEHI-5）、内皮细胞、正常人肺细胞（WI-38）、正常人成纤维细胞（D-550）、大鼠成年II型肺泡细胞（L-2）和犬肾细胞（MDCK）培养液在高压液相色谱分离前后进行放射免疫测定，来检测前列环素（以6-酮-前列环素F1α衡量）、血栓素A2（以血栓素B2衡量）以及前列腺素E2、F2α和D2的生物合成。通过这两种方法获得的每种代谢物的代谢谱和水平具有可比性。花生四烯酸生物合成谱在每种细胞中都是独特的。内皮细胞主要合成前列环素；淋巴细胞主要合成血栓素。犬肾细胞合成相对大量的前列腺素F2α、I2和E2，而正常人肺细胞主要产生前列腺素E2、F2α和血栓素。大鼠成年肺泡细胞（L-2）和正常人成纤维细胞（D-550）主要生物合成前列腺素E2和F2α。
