Sraer J, Siess W, Moulonguet-Doleris L, Oudinet J P, Dray F, Ardaillou R
Biochim Biophys Acta. 1982 Jan 15;710(1):45-52. doi: 10.1016/0005-2760(82)90188-6.
Prostaglandin synthesis by eight different structures from the rat kidney (while cortex, cortical tubules, glomeruli, outer medulla, papilla, glomerular cultured epithelial and mesangial cells, cultured interstitial medullary cells) was measured in vitro after incubation with [14C] arachidonic acid using high-performance liquid chromatography followed by RIA with four specific anti-prostaglandin antibodies (prostaglandin E2, prostaglandin F2 alpha, 6 keto-prostaglandin F1 alpha, thromboxane B2). Prostaglandin production by the whole cortex and cortical tubules was very low. The order of abundance for isolated glomeruli was thromboxane B2 great than prostaglandin E2 greater than prostaglandin F2 alpha greater than 6 keto-prostaglandin F1 alpha. Mesangial cells synthesized prostaglandin E2 at a markedly high rate, in decreasing order: prostaglandin F2 alpha, thromboxane B2 and 6 keto-prostaglandin F1 alpha. The same order of abundance was observed for epithelial cells. The papilla synthesized essentially prostaglandin E2 and prostaglandin F2 alpha, whereas the main product for the outer medullar was 6 keto-prostaglandin F1 alpha. Cultured interstitial cells synthesized mainly prostaglandin E2 and to a lesser extent prostaglandin F2 alpha. Unidentified peaks eluting between 6 keto-prostaglandin F1 alpha and thromboxane B2 were also observed chiefly with glomeruli but they were absent with the medullary preparations. They disappeared after incubation with indomethacin or aspirin and represented for glomeruli the greatest percentage of conversion of [14C] arachidonic acid. These results show that the prostanoid profile varies markedly with the different regions and cells of the rat kidney.
用高效液相色谱法，继以用四种特异性抗前列腺素抗体（前列腺素E2、前列腺素F2α、6-酮-前列腺素F1α、血栓素B2）进行放射免疫分析，在体外测定了大鼠肾脏的八种不同结构（皮质、皮质小管、肾小球、外髓质、乳头、培养的肾小球上皮细胞和系膜细胞、培养的间质髓质细胞）与[14C]花生四烯酸孵育后的前列腺素合成。整个皮质和皮质小管产生的前列腺素非常少。分离出的肾小球中前列腺素生成量的顺序为：血栓素B2＞前列腺素E2＞前列腺素F2α＞6-酮-前列腺素F1α。系膜细胞以明显高的速率合成前列腺素E2，依次递减为：前列腺素F2α、血栓素B2和6-酮-前列腺素F1α。上皮细胞也观察到相同的生成量顺序。乳头主要合成前列腺素E2和前列腺素F2α，而外髓质的主要产物是6-酮-前列腺素F1α。培养的间质细胞主要合成前列腺素E2，其次合成少量的前列腺素F2α。在6-酮-前列腺素F1α和血栓素B2之间洗脱的未鉴定峰也主要在肾小球中观察到，但在髓质制剂中不存在。与吲哚美辛或阿司匹林孵育后它们消失，并且对于肾小球而言，它们占[14C]花生四烯酸转化的最大百分比。这些结果表明，前列腺素类物质的分布在大鼠肾脏的不同区域和细胞中显著不同。
