Havemann K, Gassel W D, Laukel H
Acta Haematol. 1979;62(5-6):306-14. doi: 10.1159/000207594.
The kinetic of production of colony-stimulating activity (CSA) inducing mouse and human colony-forming cells (CFU-C) was tested in different human leukocyte culture systems. Stimulated and unstimulated cultures of spleen single cell suspensions, peripheral mononuclear leukocytes and acute monocytic leukemia (AMoL) cells were investigated. With the exception of the AMoL cells, stimulated cultures always revealed higher CSA levels than unstimulated controls. The spleen cell cultures exhibited the highest overall activity showing three molecular species of 70,000, 35,000 and 10,000 daltons activating human CFU-C to form colonies in the agar culture system. Furthermore it could be demonstrated that colony formation could be inhibited by low molecular weight fibrinogen degradation products obtained by digestion of fibrinogen with granulocyte-derived elastase.
在不同的人白细胞培养系统中测试了诱导小鼠和人集落形成细胞（CFU-C）的集落刺激活性（CSA）的产生动力学。研究了脾单细胞悬液、外周血单核白细胞和急性单核细胞白血病（AMoL）细胞的刺激培养和未刺激培养。除AMoL细胞外，刺激培养的CSA水平总是高于未刺激的对照。脾细胞培养物表现出最高的总体活性，显示出三种分子量分别为70,000、35,000和10,000道尔顿的分子物种，它们在琼脂培养系统中激活人CFU-C形成集落。此外，可以证明，用粒细胞源性弹性蛋白酶消化纤维蛋白原得到的低分子量纤维蛋白原降解产物可抑制集落形成。
