猪流行性腹泻病毒在猪细胞系中的增殖
The propagation of a porcine epidemic diarrhea virus in swine cell lines.
作者信息
Kadoi K, Sugioka H, Satoh T, Kadoi B K
机构信息
Laboratory of Bio-medical Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
出版信息
New Microbiol. 2002 Jul;25(3):285-90.
A strain of porcine epidemic diarrhea virus (PEDV), P-5V, utilized as a live virus vaccine in Japan was infected to a swine cell lines, KSEK6 and IB-RS-2 cells. Clear CPE, characterized by cellular destruction, started to appear in the infected cells on 2-3 days post infection (DPI) and affected cells was completely degenerated on 4 DPI. The virus was serially passaged in the cells even without addition of trypsin. Small but clear plaques were formed under an agar overlay medium on the cells. The infective titer in the order of 10(7.00-7.50) TCID50 per ml was obtained at usual incubation temperature.
一种在日本用作活病毒疫苗的猪流行性腹泻病毒（PEDV）毒株P-5V，被接种到猪细胞系KSEK6和IB-RS-2细胞中。感染后2 - 3天（DPI），受感染细胞开始出现以细胞破坏为特征的明显细胞病变效应（CPE），4 DPI时受影响的细胞完全退化。即使不添加胰蛋白酶，该病毒也能在细胞中连续传代。在细胞的琼脂覆盖培养基下形成了小而清晰的蚀斑。在通常的培养温度下，获得了每毫升约10(7.00 - 7.50) TCID50的感染滴度。
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