Berrocal Maria J, Johnson R Daniel, Badr Ibrahim H A, Liu Mingdong, Gao Dayong, Bachas Leonidas G
Department of Chemistry and Center of Membrane Sciences, University of Kentucky, Lexington 40506-0055, USA.
Anal Chem. 2002 Aug 1;74(15):3644-8. doi: 10.1021/ac025604v.
The hydrogelpoly(2-methacryloyloxyethylphosphorylcholine-co-butyl methacrylate), or poly(MPC-co-BMA), was used as a coating for polyurethane- and poly(vinyl chloride)-based membranes to develop ion-selective electrodes (ISEs) with enhanced blood compatibility. Adverse interactions of poly(MPC-co-BMA) with blood were diminished due to the phosphorylcholine functionalities of the hydrogel, which mimic the phospholipid polar groups present on the surface of many cell membranes. As demonstrated by immunostaining, hydrogel-coated PVC membranes soaked in platelet-rich plasma showed less adhesion and activation of platelets than uncoated PVC membranes, indicating an improvement in biocompatibility owing to the hydrogel. Furthermore, little differences in the potentiometric response characteristics, e.g., slope, detection limit, and selectivity, of ISEs employing uncoated and coated membranes were observed.
水凝胶聚（2-甲基丙烯酰氧基乙基磷酰胆碱-共-甲基丙烯酸丁酯），即聚（MPC-共-BMA），被用作基于聚氨酯和聚氯乙烯的膜的涂层，以开发具有增强血液相容性的离子选择性电极（ISE）。聚（MPC-共-BMA）与血液的不良相互作用因水凝胶的磷酰胆碱官能团而减少，这些官能团模仿了许多细胞膜表面存在的磷脂极性基团。免疫染色表明，浸泡在富含血小板血浆中的水凝胶涂层PVC膜比未涂层的PVC膜显示出更少的血小板粘附和活化，这表明水凝胶改善了生物相容性。此外，观察到使用未涂层和涂层膜的ISE的电位响应特性，如斜率、检测限和选择性，几乎没有差异。
