多糖和单糖与包覆在银纳米颗粒上的硫醇硼酸的络合作用。
Complexation of polysaccharide and monosaccharide with thiolate boronic acid capped on silver nanoparticle.
作者信息
Zhang Jian, Geddes Chris D, Lakowicz Joseph R
机构信息
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry, 725 West Lombard Street, Baltimore, MD 21201, USA.
出版信息
Anal Biochem. 2004 Sep 15;332(2):253-60. doi: 10.1016/j.ab.2004.05.051.
Abstract
Synthesized thiolate boronic acid was found to complex with both a polysaccharide (dextran) and a monosaccharide (glucose) with similar affinities but displayed more affinity with dextran than with glucose when capped as a ligand on silver nanoparticle. Coupling on multiple sites of dextran, the silver particles were aggregated. The aggregated particles displayed a decrease of absorbance at 397 nm and an increase at 640 nm. Luminescence intensity displayed an upward deviation increase with the concentration of dextran. The luminescence spectral change was ascribed to surface-enhanced fluorescence by the enhanced field from the aggregated metallic particles.
摘要
合成硫醇盐硼酸与多糖（葡聚糖）和单糖（葡萄糖）形成复合物时具有相似的亲和力，但当作为配体封端在银纳米颗粒上时，其对葡聚糖的亲和力比对葡萄糖的亲和力更高。银颗粒在葡聚糖的多个位点上偶联，从而发生聚集。聚集的颗粒在397nm处的吸光度降低，在640nm处的吸光度增加。发光强度随葡聚糖浓度的增加呈向上的偏差增加。发光光谱的变化归因于聚集金属颗粒增强场导致的表面增强荧光。
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