Sando Shinsuke, Kool Eric T
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
J Am Chem Soc. 2002 Aug 21;124(33):9686-7. doi: 10.1021/ja026649g.
We report on the application of a new class of oligonucleotide reporter probes, QUAL probes, that "light up" when a nucleophilic phosphorothioate probe binds adjacent to a dabsyl-quenched probe. These self-ligating DNA probes were used for sequence-specific detection of 16S rRNA in Escherichia coli cells. Strong fluorescence was observed only when the phosphorothioate and quenched dabsyl probes bind side-by-side on a 16S rRNA target. The results demonstrate the use of QUAL probes to detect specific RNA sequences in bacterial cells without enzymes and without washing steps.
我们报道了一类新型寡核苷酸报告探针——QUAL探针的应用，当亲核硫代磷酸酯探针与达布酰淬灭探针相邻结合时，该探针会“发光”。这些自连接DNA探针用于大肠杆菌细胞中16S rRNA的序列特异性检测。只有当硫代磷酸酯探针和淬灭的达布酰探针在16S rRNA靶标上并排结合时，才会观察到强烈的荧光。结果表明，QUAL探针可用于在无需酶和洗涤步骤的情况下检测细菌细胞中的特定RNA序列。
