Mito Taro, Inoue Yoshiko, Kimura Shinsuke, Miyawaki Katsuyuki, Niwa Nao, Shinmyo Yohei, Ohuchi Hideyo, Noji Sumihare
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, 2-1 Minami-Jyosanjima-cho, Tokushima City 770-8506, Japan.
Mech Dev. 2002 Jun;114(1-2):27-35. doi: 10.1016/s0925-4773(02)00052-7.
To understand the mechanism of regeneration, many experiments have been carried out with hemimetabolous insects, since their nymphs possess the ability to regenerate amputated legs. We first succeeded in observing expression patterns of hedgehog, wingless (wg), and decapentaplegic (dpp) during leg regeneration of the cricket Gryllus bimaculatus. The observed expression patterns were essentially consistent with the predictions derived from the boundary model modified by Campbell and Tomlinson (CTBM). Thus, we concluded that the formation of the proximodistal axis of a regenerating leg is triggered at a site where ventral wg-expressing cells abut dorsal dpp-expressing cells in the anteroposterior (A/P) boundary, as postulated in the CTBM.
为了了解再生机制，人们对渐变态昆虫进行了许多实验，因为它们的若虫具有再生被切断腿部的能力。我们首次成功观察到了双斑蟋腿部再生过程中刺猬蛋白、无翅基因（wg）和果蝇的decapentaplegic基因（dpp）的表达模式。观察到的表达模式与由坎贝尔和汤姆林森修改后的边界模型（CTBM）得出的预测基本一致。因此，我们得出结论，正如CTBM所假设的那样，再生腿部近远轴的形成是在前后（A/P）边界处腹侧表达wg的细胞与背侧表达dpp的细胞相邻的部位触发的。
