植物病毒检测的简易方法：抗体固定技术的效果
Simple method for plant virus detection: effect of antibody immobilization technique.
作者信息
Boltovets P M, Boyko V R, Kostikov I Yu, Dyachenko N S, Snopok B A, Shirshov Yu M
机构信息
D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences, 145 Zabolotny St, Kyiv 03143, Ukraine.
出版信息
J Virol Methods. 2002 Aug;105(1):141-6. doi: 10.1016/s0166-0934(02)00098-8.
The possibility has been demonstrated for applying a surface plasmon resonance for detecting plant viruses in real samples. An optimal mode for antiviral immunoglobulin immobilization on sensor surfaces is described. Out of three proposed techniques for sensor surface treatment, namely, unmodified gold surface, gold surface treated with (a) thiocyanate and (b) thiocyanate and protein A (Staphylococcus aureus), the latter was chosen as most suited for retention of the formed native immunoglobulin layer.
已证明表面等离子体共振可用于检测实际样品中的植物病毒。描述了抗病毒免疫球蛋白固定在传感器表面的最佳模式。在三种提出的传感器表面处理技术中，即未修饰的金表面、用（a）硫氰酸盐和（b）硫氰酸盐及蛋白A（金黄色葡萄球菌）处理的金表面，后者被选为最适合保留形成的天然免疫球蛋白层的技术。
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