V. Ye. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine, Prospekt Nauki, 41, 03028 Kyiv, Ukraine.
Analyst. 2013 Jan 21;138(2):480-6. doi: 10.1039/c2an35972c.
Here, we describe a highly sensitive method that allows for the correct quantification of inhibition effect with a higher degree of accuracy directly at the molecular level. The protocol involves two stages, namely serological virus titration in comparison with the same procedure for virus-effector mixture. Owing to the robustness of the analysis this assay can be performed on crude cellular and plant extracts, and therefore it may be suitable for the routine analysis of clinical samples, or in the field. The efficiency of the approach to the quantification of the inhibition effect of polysaccharide glucuronoxylomannan (GXM) on the infection efficiency of the tobacco mosaic virus (TMV) was investigated using advanced serological approaches based on label-free surface plasmon resonance technique. It was shown that GXM drastically decreases the efficiency of TMV infection by blocking up to 70% of the virus shell. The obtained results are in conformity with the method of indicator plant infection.
在这里，我们描述了一种高度敏感的方法，可在分子水平上直接更准确地定量抑制效果。该方案包括两个阶段，即与病毒效应物混合物的相同程序进行血清学病毒滴定。由于分析的稳健性，该测定法可以在粗细胞和植物提取物上进行，因此它可能适用于临床样品的常规分析，或在现场进行。使用基于无标记表面等离子体共振技术的先进血清学方法研究了多糖葡糖醛酸木聚糖（GXM）对烟草花叶病毒（TMV）感染效率的抑制效果的定量方法的效率。结果表明，GXM 通过阻止多达 70%的病毒壳，大大降低了 TMV 的感染效率。所获得的结果与指示植物感染的方法一致。
