Baac Hyoungwon, Hajós József P, Lee Jennifer, Kim Donghyun, Kim Sung June, Shuler Michael L
Department of Biomedical Engineering, Cornell University, Ithaca, New York 14853, New York, USA.
Biotechnol Bioeng. 2006 Jul 5;94(4):815-9. doi: 10.1002/bit.20882.
Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two-channel (reference & test) micro-fluidic SPR system is used for reliable measurement. Bio-specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors.
表面等离子体共振（SPR）技术被用于直接检测昆虫病原体的完整形式：杆状病毒，苜蓿银纹夜蛾多核多角体病毒（AcMNPV）。使用具有三个生物功能层的SPR传感器芯片来检测完整的AcMNPV：带有二硫键的胺反应性交联剂，其化学吸附到金膜上、蛋白A以及针对目标病毒病原体表面蛋白产生的小鼠IgG单克隆抗体。一个双通道（参考通道和测试通道）微流体SPR系统用于进行可靠的测量。将对AcMNPV的生物特异性响应与作为对照的烟草花叶病毒（TMV）的响应进行比较。传感器芯片先后暴露于两种病毒，验证了对AcMNPV的特异性响应。这是开发新型病毒病原体检测传感器的先决条件。
