Liu Fang, Sun Gen-Lou, Salomon Bjorn, von Bothmer Roland
Department of Crop Science, The Swedish University of Agricultural Sciences, Alnarp.
Hereditas. 2002;136(1):67-73. doi: 10.1034/j.1601-5223.2002.1360110.x.
Genetic variability in the 143 core accessions of wild barley, Hordeum vulgare ssp. spontaneum, was assessed by allozyme analysis. A total of 34 alleles were detected at ten isozyme loci. All loci were polymorphic except Pgd-1, which was monomorphic. Est-2 and Est-4 were the most diverse loci, with genetic diversity values of 0.747 and 0.686, respectively. The comparison of the results with those of previous studies indicates that all alleles occurring in cultivated and wild barley are observed in this set of the wild Barley Core Collection. Only one allele (Pgd-1 Tj) was absent. It is noteworthy that one new allele at the Ndh-2 locus and another new allele at Aco-2 locus were first detected in the present study. Nine of the 34 alleles were rare and detected only in one to four accessions. The genetic similarities among the 143 accessions ranged from 0.18 to 1.00. Data analysis based on clustering and principal coordinate analysis showed that a high level of genetic variability exists in this set of core accessions, and indicated that some duplication probably exists in this set core based on the present study.
通过等位酶分析评估了野生大麦（Hordeum vulgare ssp. spontaneum）143份核心种质的遗传变异性。在10个同工酶位点共检测到34个等位基因。除单态的Pgd - 1外，所有位点均为多态性。Est - 2和Est - 4是多样性最高的位点，遗传多样性值分别为0.747和0.686。将结果与先前研究结果进行比较表明，在这组野生大麦核心种质中观察到了栽培大麦和野生大麦中出现的所有等位基因。仅缺少一个等位基因（Pgd - 1 Tj）。值得注意的是，本研究首次在Ndh - 2位点检测到一个新等位基因，在Aco - 2位点检测到另一个新等位基因。34个等位基因中有9个罕见，仅在1至4份种质中检测到。143份种质之间的遗传相似性范围为0.18至1.00。基于聚类和主坐标分析的数据分析表明，这组核心种质存在高水平的遗传变异性，并表明基于本研究，这组核心种质中可能存在一些重复。
