用于研究结直肠癌吻合口周围复发的实验模型
Experimental model for the study of perianastomotic recurrence in colorectal cancer.
作者信息
Noguera Aguilar J F, Tortajada Collado C, Morón Canis J M, Plaza Martínez A, Amengual Antich I, Pujol Tugores J J
机构信息
Service of General Surgery, Fundación Hospital Son Llátzer, Son Ferriol, Baleares, Spain.
出版信息
Rev Esp Enferm Dig. 2002 Mar;94(3):131-8.
OBJECTIVE
To determine if an experimental model is valid for the study of perianastomotic recurrence in colorectal cancer, comparing it with previous experimental models.
METHODS
Experimental study with 40 male Sprague-Dawley rats, assigned to one of the study groups: control group (n = 20), with manipulation of large descending bowel, and colonic anastomosis group (n = 20), with colonic section and colocolic anastomosis. After pharmacological carcinogenesis with 1-2 dimethylhydrazine at a weekly dose of 25 mg/kg for 18 weeks, colonic tumours were studied at the 20th postoperative week.
RESULTS
Number of tumours, colic tumoral area and percentage of colic tumoral area were greater in the colonic anastomosis group. In this group with colonic anastomosis all determinations were higher at the perianastomotic large bowel.
CONCLUSIONS
We think this experimental model may be the best model to study perianastomotic recurrence in large bowel cancer. The high incidence of induced colic tumours and their location at the perianastomotic area offer a good field to determine response to experimental manipulations on colorrectal cancer.
目的
通过与既往实验模型比较，确定一种实验模型是否适用于研究结直肠癌吻合口周围复发情况。
方法
对40只雄性斯普拉格-道利大鼠进行实验研究，将其分为以下研究组之一：对照组（n = 20），对降结肠进行操作；结肠吻合组（n = 20），进行结肠切断和结肠结肠吻合。在每周给予1-2二甲基肼25 mg/kg，共18周的药物致癌作用后，于术后第20周对结肠肿瘤进行研究。
结果
结肠吻合组的肿瘤数量、结肠肿瘤面积和结肠肿瘤面积百分比更高。在这个结肠吻合组中，吻合口周围大肠的所有测定值都更高。
结论
我们认为该实验模型可能是研究大肠癌吻合口周围复发的最佳模型。诱导产生的结肠肿瘤的高发生率及其在吻合口周围区域的位置，为确定对结直肠癌实验操作的反应提供了一个良好的研究领域。
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